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Bal31 PFGE and Southern Blot. HMW genomic DNA from An. albimanus pupae was digested with Bal31 exonuclease at time point increments followed by restriction enzyme digestion with XbaI then separated by Pulsed-Field Gel Electrophoresis (left). The separated DNA was transferred to charged Nylon membrane via downward capillary action, hybridized with a DIG- labeled telomeric probe, and detected using chemiluminescence (right).
