Figure 1.
Whole genome alignment and structural confirmation using optical mapping. A. Dotplot showing a comparison between AF13 and NRRL3357. A large insertion (310 Kb) can be observed on Chromosome 1. B. Comparison between AF13 and A. oryzae RIB40 at the insert position (enlarged in C) clearly showed alignment to a region on A. oryzae Chromosome 8 for the insertion. Otherwise, the genomes shared a similar structure with the exception of a translocation on Chromosomes 6 and 2. D. Bionano optical mapping reads (blue) aligned to assembled PacBio contigs (green) show sufficient read depth in the region to confirm the presence of the insertion and validate the AF13 assembly.