Table 3. Genotype comparison of 13 samples that were genotyped with the KASP assay and sequencing at positions 14,978,562, 14,978,613, and 14,979,298 on Chromosome 1 from the Rubus occidentalis v3.0 genome assembly. Eleven of the 13 samples had the same genotypes with both methods while two samples, ORUS 4674C and A-2487T, had homozygous genotypes of the targeted allele at two and three of the positions investigated, respectively.
| Sample Name | Ro01 14,978,562 | Ro01 14,978,613 | Ro01 14,979,298 | |||
|---|---|---|---|---|---|---|
| Sequencing | KASP | Sequencing | KASP | Sequencing | KASP | |
| ORUS 4540A | A:T | A:T | AC:GT | AC:GT | T:A | T:A |
| ORUS 4647U | A:A | A:A | AC:AC | AC:AC | T:T | T:T |
| ORUS 4674C | A:T | T:T | AC:GT | GT:GT | T:A | T:A |
| Marion | A:T | A:T | AC:GT | AC:GT | T:A | T:A |
| Nightfall | A:A | A:A | AC:AC | AC:AC | T:T | T:T |
| Waldo | A:G | A:A | AC:AC | AC:AC | T:T | T:T |
| Bassettberry | A:G | A:A | AC:AA | AC:AC | T:T | T:T |
| ORUS 4647L | A:A | A:A | AC:AC | AC:AC | T:T | T:T |
| ORUS 4647M | A:A | A:A | AC:AC | AC:AC | T:T | T:T |
| ORUS 4647R | A:A | A:A | AC:AC | AC:AC | T:T | T:T |
| Silvan | A:A | A:A | AC:AA | AC:AC | T:T | T:T |
| Osage | A:T | A:T | AC:GT | AC:GT | T:A | T:A |
| A-2487T | A:T | T:T | AC:GT | GT:GT | T:A | A:A |