Table 1. Photophysical Properties of Synthetic SNAP Dyes and Cellular Uptake in BY-2 Cells.
| Compound | Permeability | λabs (nm) | λem (nm) | φfl | Application | Reference, Catalog No. and Supplier |
|---|---|---|---|---|---|---|
| SNAP-Cell 430 | + | 421 | 444, 484 | Covalent labeling | S9109S, NEB | |
| SNAP-Surface Alexa Fluor 488 | - | 496 | 520 | covalent labeling | S9129S, NEB | |
| DRBG-488 | n.d.a | 496 | 520 | covalent labeling, pulse chase labeling | (Komatsu et al., 2011) | |
| SNAP-Cell TMR-Star | + | 554 | 580 | covalent labeling | S9105S, NEB | |
| SNAP-Cell 647SiR | + | 645 | 661 | covalent labeling | S9102S, NEB |
Plus (+) and minus (-) indicate intracellular/extracellular signal intensity at the 1-min time point >0.4 and <0.2, respectively. λabs, absorption maximum; λem, fluorescent emission maximum; φfl, fluorescence quantum yield. n.d., not determined. Blank cells indicate no data.
a
DRBG-488 is not fluorescent until reaction with a SNAP-tag.