Figure 2.

Identification of the p3d3 Causal Mutation through Mapping-by-Sequencing.

(A) Distribution of the allelic frequencies of EMS mutations in the wild-type (WT)–like and mutant-like bulks are represented along the tomato genome in sliding windows of 30 consecutive mutations. In the case of a recessive mutation, allelic frequency of the causal mutation/region should be 1 and 0.33 in the mutant-like and wild-type–like bulks, respectively. Ch, chromosome.

(B) The mutations used as markers for recombination analysis are indicated along Ch10. The blue box represents the causal 1.51-Mb region encompassing 131 genes.

(C) Solyc10g008950 is the only gene containing an EMS-induced mutation in the causal region. The mutation corresponds to a T deletion in exon 2 (blue box) of Solyc10g008950. Exons are represented by boxes.