Figure 3.

Genetic and Cytological Analysis of Osrdr6-bi.

(A) Mutation sites of Osrdr6-mei, Osrdr6-edi, and Osrdr6-bi.

(B) Meiotic chromosome behavior of meiocytes from the wild-type (WT) and Osrdr6-bi plants. Bars = 5 μm.

(C) Proportion of meiocytes at different stages in 8-mm anthers from the wild type (WT) and Osrdr6-bi.

(D) Transverse sections of the anthers from the wild type (WT) and Osrdr6-bi. Images are cross sections of a single locule. Bars = 20 μm.

(E) Dual immunolocalization of PAIR3 (red) and ZEP1 (green) in wild-type (WT) and Osrdr6-bi meiocytes. PAIR3 was used to visualize the meiotic chromosomes. Bars = 5 μm.

(F) Dual immunolocalization of PAIR3 (green) and γH2AX (red) in WT and Osrdr6-bi meiocytes. Bars = 5 μm.