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. 2020 Aug 6;32(10):3346–3369. doi: 10.1105/tpc.20.00027

Figure 6.

Figure 6.

Trichomes of glcat14a/b and glcat14a/b/d Mutants Have Reduced Numbers of Branches.

(A) and (B) CryoSEM micrographs of trichomes of the wild type, glcat14a, glcat14b, glcat14d-1, and glcat14e (A) and the wild type, glcat14a/b, glcat14a/b/d, and glcat14a/b/e (B) third true leaves from plants grown on basal MS medium. Bars = 100 μm.

(C) Quantification of trichome branching points from third true leaves from the wild type and glcat14 mutants. The percentage of trichomes by number of branching points was calculated. NA, data not available as trichomes did not branch. The graph represents means ± sd of three biological replicates. n = 10 leaves per line per replicate. Data points are shown for trichomes with one and two branching points. Asterisks indicate significant differences between mutants and the wild type defined by one-way ANOVA followed by Tukey’s multiple comparison test: ****, P < 0.0001.

(D) Genetic complementation of glcat14a/b/d-1. Third true leaves of GLCAT14B-complemented lines #1 and #2 show normal trichome branching when compared with glcat14a/b/d-1. Bar = 1 mm.