Fig. 2. NO₂-OA prevents Western diet-induced atherosclerosis in Apoe^−/− mice.

Male Apoe^−/− mice were fed standard chow diet or Western diet (Envigo TD.88137, 42% fat and 0.2% cholesterol) for 8 weeks and treated with 8 mg/kg/day of NO₂-OA via subcutaneously implanted osmotic mini-pumps. Mice fed chow diet and administrated vehicle (polyethylene glycol) as well as mice fed Western diet and administrated vehicle or 8 mg/kg/d of non-nitrated OA were served as controls. Oil Red O staining was used for en face analysis of atherosclerotic lesions in the aortic tree. Representative aortas from each experimental group are shown. Statistical differences were compared by one-way ANOVA followed by Bonferroni post-hoc test (n=8 mice per group).