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. 2020 Jun 30;128(6):067015. doi: 10.1289/EHP6263

Figure 3.

Figure 3 includes two graphs. First graph plots difference in percent methylation, 95 percent CI, ranging from negative 10 to 20 in increments of 10 (y-axis) across DMR (x-axis). There is a scale titled position, ranging from 2.32 megabase to 2.335 megabase in increments of 0.005. Ensembl annotations, namely, C11orf21 and TSPAN32 are represented by two arrows pointing toward left and right, respectively. Second graph plots difference in percent methylation, 95 percent CI, ranging from negative 10 to 20 in increments of 10 (y-axis) across DMR (x-axis). There is a scale titled position, ranging from 2.322 megabase to 2.323 megabase in increments of 0.0005. Ensembl annotations, namely, TSPAN32, C11orf21 exon, and TSPAN32 exon are marked in the map and represented by arrows. A scale titled Chromosome 11 is present at the top of the two graphs.

Epigenome-wide association results in the genomic region containing the differentially methylated region (DMR) chr11:2,322,050-2,323,247 (shaded area). The first panel shows the region from 7,000 nucleotides upstream of the DMR to 17,000 nucleotides downstream of the DMR; the second panel shows the region from 300 nucleotides upstream of the DMR to 300 nucleotides downstream of the DMR. Difference in % methylation from limma models of Beta-values [i.e., mean difference in percent methylation for each unit change in ln(total urinary arsenic levels)] adjusted for age, sex, BMI, self-reported smoking status, education (<high school, high school graduate or GED, >high school), study center (Arizona, Oklahoma, North and South Dakota), estimated glomerular filtration rate, and cell type proportion estimates. Trend in odds of methylation related to As levels is indicated by a solid blue line fitted using polynomial splines. Note: BMI, body mass index; GED, General Education Diploma.