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. 2020 Aug 27;21(10):e48483. doi: 10.15252/embr.201948483

Figure 4. miR‐195 regulates clonal growth by targeting MICU1.

Figure 4

  • A, B
    Stable cell lines for CP20 and OVCAR4 expressing GFP‐miR-195 or non‐target miR‐GFP were generated using lentiviral‐mediated transduction and were selected using puromycin; representative images are shown. Scale bars, 50 μm.
  • C
    Relative miR‐195 expression was measured using RT–qPCR in stable cell lines, normalized with U6. Mean ± SEM; n = 3, biological repeats. *P < 0.05, Student's t‐test.
  • D
    CP20 and OVCAR4 stable cell lines were transfected as either empty vector (EV) or pLYS1‐MICU1‐Flag (MICU1) and relative MICU1 levels were evaluated using immunoblotting.
  • E, F
    CP20 and OVCAR4 stable cell lines transfected as in (D) were counted and re‐plated for anchorage‐dependent (E) and independent clonal growth (F). Quantification compared to the miR‐CTL stable cells transfected with EV is shown. Mean ± SD; n = 3, Biological repeats. *P < 0.05 when comparing with miR‐CTL, # P < 0.05 when comparing with miR‐195 by Student's t‐test.