Figure EV4. miR‐195 overexpression does not alter cytosolic Ca²⁺, MCU complex protein expression, and mitochondrial morphology in ovarian cancer.

• A, B
  After miR‐CTL or miR‐195 transfection in CP20 cells cytosolic Ca²⁺ was measured (A) Fluorescence traces (Fluo‐4 AM) vs time (s) showing Tg (4 μM) induced characteristic increase in cytosolic Ca²⁺ (5 mM) and uptake and retention of SOC activity following Ca²⁺ addition in both miR‐CTL and miR 195 transfected cells. Tg and Ca²⁺ were added as indicated. (B) Quantification of normalized peak fluorescence (Fluo‐4 AM) after Tg stimulation and Ca²⁺ addition. Non‐parametric t‐test determined the level of significance of fluorescence between miR‐CTL and miR‐195-treated cells (n = 3, biological repeats), (ns = not significant, Student's t‐test).
• C
  After miR‐CTL or miR‐195 transfection in OVCAR4, OVSAHO, and CP20 cells, cell lysate was evaluated for the expression of MFN2, MCU, MICU1, MICU2, and EMRE. β‐Actin is used as loading control.
• D
  Immunofluorescence of CP20 and OVCAR4 cells labeled with Mitotracker red (Scale bar 5 μM), inset shows enlarged image portion (Scale bar 2 μM).