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. 2020 Aug 27;21(10):e48483. doi: 10.15252/embr.201948483

Figure EV5. MICU1 stable cell line and toxicity assessment in miR‐CTL and miR‐195 tumor model.

Figure EV5

  • A, B
    MICU1 was stably expressed in miR stable transfected CP20 cells by using pLenti‐C-HA-IRES‐BSD plasmid (OriGene Technologies); MICU1 stable cells were selected using Blasticidin. MICU1 expression in stable cells is shown by IFC (A) and immunoblotting (B).
  • C, D
    Toxicity in experimental mice caused by miR‐195 overexpressing CP20 cells was assessed by changes in body weight and histology of vital organs. (C) Average body weight of mice from miR‐CTL and miR‐195 experimental groups immediately before euthanasia. Mean ± SD, n = 10 is shown. (D) Representative H&E‐stained sections of tissues (liver, spleen, kidneys, lungs, and heart) from miR‐CTL and miR‐195 mice visualized using an upright epi‐fluorescent microscope (Nikon NI‐U, Plan Apochromatic). Scale bar 25 μM.