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. 2020 Sep 1;21(10):e49332. doi: 10.15252/embr.201949332

Figure 6. TLR4‐induced signaling pathways were inhibited in Atx‐ko macrophages.

Figure 6

  • A–C
    The macrophages from AtxΔΜΕ/ΔΜΕ and Atx+/+ mice (A, B) or Atx‐heterozygous (Atx‐Het) mice and wild‐type littermates (C) were stimulated by LPS [20 ng/ml, 20 min (A, B) or indicated time point (C)].
  • D
    Raw264.7 cells were stimulated by LPS with the ATX inhibitor.
  • E–H
    The macrophages from AtxΔΜΕ/ΔΜΕ mice and Atx+/+ littermates were activated with TLR2 ligand Peptidoglycan (PGN, 30 ng/ml) (E), Pam3CSK4 (1 μg/ml) (F), IL‐1β (100 ng/ml) (G), or TNFα (25 ng/ml) (H) for the indicated time points.
Data information: The cell lysates were subjected to immunoblotting analysis with antibodies as indicated. Regular ERK1/2, regular Akt, β‐Actin, or RasGAP levels were used for a loading control. Presented is the representative image from at least three independent experiments. Source data are available online for this figure.