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. 2020 Aug 12;21(10):e50718. doi: 10.15252/embr.202050718

Figure EV2. Impact of MRE11 depletion on XRA‐SASP.

Figure EV2

  • A
    BJ fibroblasts were infected with lentiviruses expressing shGFP.3 or shMRE11.5, selected with puromycin and allowed to recover for 7 days. BJ‐shGFP.3 and BJ‐shMRE11.5 cells were untreated (control) or irradiated with 10 Gy of XRA. After 9 days, serum‐free conditioned medium (SF‐CM) was collected over 24 h. IL‐6 secretion in SF‐CM was analyzed by ELISA. Data are the means ± SD of triplicates and representative of two independent experiments.
  • B
    Secreted soluble factors were evaluated using a multiplex immunoassay (40‐Plex MSD®). Average secretion of untreated BJ‐shGFP.3 cells was used as baseline. Heat map key indicates log2‐fold changes from control. Unpaired t‐test: **P < 0.01.