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. 2020 Sep 18;21(10):e49585. doi: 10.15252/embr.201949585

Figure EV1. Protein aggregation caused by enzymatic degradation of RNA, and effect of pre‐hydrolysed RNA or RNase inhibition.

Figure EV1

  • A
    SDS–PAGE analysis of soluble (Supernatant) and aggregated (Pellet) proteins after treatment of mouse brain tissue lysate with increasing amounts of a mixture of RNase A and T1 (A/T1) or vehicle (Ve−).
  • B
    Agarose gel electrophoresis analysis of RNA isolated from RNase‐treated human neuronal cell lysate.
  • C, D
    Insoluble proteins collected by centrifugation after co‐treatment of human neuronal cell lysate with RNase A and an RNase A inhibitor (RNasin, C), or following the addition of RNA pre‐hydrolysed by RNase A or alkaline hydrolysis (NaOH, D).