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. 2020 Sep 18;21(10):e49585. doi: 10.15252/embr.201949585

Figure 3. RNA is required for renaturation and function of proteins aggregated by enzymatic degradation of RNA in human neuronal lysates.

Figure 3

  • A
    Diagram showing the renaturing assay.
  • B
    Effect of RNA/protein ratio on protein aggregation after renaturing.
  • C
    Coomassie‐stained gel of soluble (Sup 1/2) and aggregated (Pel 1/2) proteins after removal of GuHCl in the presence (+) or absence (−) of total RNA. Asterisk (*) denotes added RNase A.
  • D
    As in (C) but analysed by Western blot.
  • E
    ATP‐hydrolysing activity of proteins renatured with total RNA or buffer (Ve−) after removal of GuHCl.
Data information: All experiments were performed with total RNA. Data in (B and E) are expressed in arbitrary units (AU) and represent the mean ± s.d of two (B) or three (E) independent experiments. **P < 0.01, *P < 0.05 by post hoc ANOVA.