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. 2020 Sep 18;21(10):e49585. doi: 10.15252/embr.201949585

Figure 5. Renaturing characteristics of selected motifs.

Figure 5

  • A
    Proportion of aggregated proteins after renaturation with either single‐stranded (Forward or Reverse) or complementary strands (For/Rev) of the selected M1x4 or the control oligonucleotide, M2x4.
  • B
    Western blot analysis of proteins renatured with either single‐stranded or double‐stranded M1x4 or M2x4 DNA oligonucleotides.
  • C
    The effect on protein re‐aggregation from varying the number of DNA motif repeats when complementary oligonucleotides are given together.
  • D
    Protein aggregation after renaturation with the M1x4 DNA oligonucleotides pre‐annealed with 100 mM NaCl or vehicle (None).
Data information: Data are expressed as a fraction of vehicle (Ve−) and represent the mean ± s.d of three to four independent experiments. **< 0.01, *0.05 by post hoc ANOVA.