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. 2020 Aug 19;21(10):e49555. doi: 10.15252/embr.201949555

Figure 2. Cells in epithelial clusters map to spatially distinct regions of the wing disc.

Figure 2

  • A
    Schematic representation of the wing imaginal disc and its relationship to the adult wing. The cell fate map of the wing disc is adapted from (Bryant, 1975).
  • B
    Subset of 6,983 epithelial cells in UMAP plot of the reference single‐cell atlas.
  • C
    Approximate map of the epithelial clusters identified in the reference single‐cell atlas dataset to distinct position over the disc proper.
  • D
    Average expression level of the genes used as known markers to assign each epithelial cluster in the reference atlas dataset. Cells coloured by the expression of nub, wg, zfh2, Sox15, grn, bnl, eyg, Ance, Idg4 and drm.
  • E
    Dot plot showing the expression levels of the top marker genes identified for the epithelial cells across 17 clusters of the reference cell atlas dataset.
  • F
    Confocal single plane image of third instar larval wing disc stained with anti‐Nub, anti‐Wg, anti‐Svp, anti‐Hairy (red), reporting expression of dpp‐lacZ, Dad‐lacZ (anti‐β‐gal, red) and counterstained with DAPI (cyan). Scale bars: 100 μm. Full genotypes: P{CaryP}attP2 (top and bottom panels), P{PZ}dpp[10638]; +, and y‐ w‐; +; P{lacW}Dad[j1E4].