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. 2020 Aug 19;21(10):e49555. doi: 10.15252/embr.201949555

Figure 4. IFM and DFM myoblast clusters uncover different state of differentiation.

Figure 4

  • A
    Dot plot showing the expression levels of the top variable genes between myoblasts across 7 clusters of the reference cell atlas dataset.
  • B–D
    Confocal single plane images of third instar larval wing discs stained with anti‐Ct (red) and (B) E(spl)m6‐BFM-gapGFP (green), (C) E(spl)m7‐HLH[VK00037]‐GFP (green), or (D) E(spl)m3‐HLH[GMR10E12]>GFP (green).
  • E–G
    Slingshot used for inferring cell lineages and pseudotime among the IFM myoblasts. (E) Two lineages for IFM myoblast clusters visualized in subset of 3,492 cells in UMAP plot of the reference single‐cell atlas dataset. (F) Pseudotime inference in Lineage 1 is from IFM_1 → IFM_2 → IFM_3 → IFM_4. (G) Pseudotime inference in Lineage 2 is from IFM_1 → IFM_2 → IFM_3 → IFM_5.
  • H, I
    Confocal single plane images of third instar larval wing discs stained with anti‐Ct (cyan) showing the lineage of GAL4 (green) and the active GAL4 (red) in (H) E(spl)m3‐HLH[GMR10E12]>gTRACE and (I) E(spl)m6‐BFM-GAL4>gTRACE.
Data information: White arrowheads point to location of IFM_1‐2 cells. Red asterisks indicate expression of E(spl)m3‐HLH in epithelial cells. Scale bars: 50 μm. Full genotypes are (B) w‐; UAS‐GFP; E(spl)m3‐HLH[GMR10E12]‐GAL4, (C) w‐; E(spl)m7‐HLH-GFP[VK00037]; +, (D) E(spl)m6‐BFM-gapGFP, (H) w‐; UAS‐gTRACE/+; E(spl)m3‐HLH[GMR10E12]-GAL4/+, (I) w‐; UAS‐gTRACE, E(spl)m6‐BFM-GAL4.Source data are available online for this figure.