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A
Lethality of 1151>Ama‐RNAi[HMS00297] animals at pharate stage was partially rescued by overexpressing the transgene Ama
OE‐cDNA. Representative images of adults and pharate pupa are shown. Full genotypes: 1151‐GAL4; UAS‐myrRFP; +, 1151‐GAL4; UAS‐myrRFP; UAS‐Ama[HMS00297]‐RNAi, 1151‐GAL4; UAS‐Ama
OE
/CyO; UAS‐Ama[HMS00297]‐RNAi and 1151‐GAL4; UAS‐Ama
OE
; +.
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B
Two‐dimensional UMAP representation of single‐cell RNA‐seq 1151>Ama‐RNAi dataset coloured by cell type, including 51 tracheal, 3,107 epithelial and 180 myoblast cells.
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C
Average expression level of the genes Fas3 (left panel) and zfh1 (right panel) in the 1151>Ama‐RNAi dataset.
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D
Confocal single plane images of 1151>mCherry‐RNAi and 1151>Ama‐RNAi wing discs stained with anti‐Ct (red) and anti‐Zfh1 (green).
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E
Dot plots showing the expression levels of the top markers for myoblast clusters identified in the reference cell atlas across the cluster of myoblasts in 1151>Ama‐RNAi and 7 myoblast clusters in control dataset.
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F
The myoblast cells in 1151>Ama‐RNAi dataset projected into the reference single‐cell atlas by transferring cell‐type labels using Seurat. The bar graph represents the total number of cells in each cluster normalized to the total number of epithelial cells per genotype.
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G, H
Confocal single plane images of 11511>mCherry‐RNAi and 1151>Ama‐RNAi wing discs stained with anti‐Zfh1 (green) and (G) anti‐Nrt (white) at wandering third instar larval stage (110–135 h AEL) or (H) DAPI (red) at early third instar larval wing discs (72–110 h AEL).
Data information: Scale bars: 50 μm. Full genotypes: (D, G, H)
.
