Skip to main content
. 2020 Aug 19;21(10):e49555. doi: 10.15252/embr.201949555

Figure 8. The loss of Amalgam impairs the formation of both IFM and DFM at early stages of developing pupa.

Figure 8

Confocal single plane images of 1151>Ama‐RNAi animals and 1151>mCherry‐RNAi.
  • A
    Forming DFM at 40 h APF stained with anti‐Zfh1 (green), anti‐Futsch (22c10, red) and anti‐Kettin (white). White arrows point to the wing hinge, wings pointing left, anterior up.
  • B
    Forming IFM (DLM) at 16 h APF, stained with anti‐Futsch (22c10, red), anti‐Zfh1 (green) and DAPI (cyan). Yellow‐dashed box indicates magnified area (bottom panel). Anterior up.
  • C
    DFM at 96 h APF stained with Phalloidin (red), anti‐Kettin (green) and DAPI (blue). Anterior up, dorsal right. DFM are numbered in white as in (Lawrence, 1982).
  • D
    IFM at 96 h APF, transverse section (top) stained with anti‐PS-integrin (green), Phalloidin (red) and DAPI (blue), dorsal up; sagittal section (bottom) stained with anti‐Kettin (green), Phalloidin (red) and DAPI (blue), anterior up, dorsal right.
Data information: Scale bars: 50 μm (A, B), and 100 μm (C, D). Full genotypes: 1151‐GAL4; +; UAS‐mCherry‐RNAi and 1151‐GAL4; +; UAS‐Ama[HMS00297]‐RNAi.