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. 2020 Aug 12;21(10):e49689. doi: 10.15252/embr.201949689

Figure 8. circLMP2A modulates the expression of miR‐3908 target TRIM59.

Figure 8

  • A
    Schematic representation of the putative binding sites for miR‐3908 and TRIM59.
  • B
    Dual‐luciferase reporter assay for the luciferase activity of the indicated plasmids (TRIM59‐Wt and TRIM59‐Mut luciferase reporter) in HEK‐293T cells transfected with mimic‐NC or miR‐3908.
  • C
    Real‐time PCR analysis of the mRNA level of TRIM59 in SNU719 and SNU‐4th cells.
  • D–F
    Real‐time PCR analysis of the expression of TRIM59 in SNU‐4th (D), SNU719 (E) and YCCEL1 cells (F) transfected with the above different mimics.
  • G–I
    WB analysis of the expression of TRIM59 and p53 in SNU‐4th (G), SNU719 (H) and YCCEL1 cells (I) transfected with the above different mimics.
  • J
    Immunohistochemical staining showing that over‐expression of circLMP2A could lead to increased expression of TRIM59 and ERG but decreased expression of p53.
  • K
    WB analysis of the expression of TRIM59 and p53 in tumour xenografts.
  • L
    Immunohistochemical staining showing that high expression of circLMP2A in EBVaGC tissues resulted in significant increases in CD44+CD24− cells and significant decreases in p53+ cells.
Data information: Results are presented as the mean ± SD, n = 3 biological replicates, scale bars = 50 μm, *P < 0.05; **P < 0.01; ***P < 0.001, Student's t‐test. Source data are available online for this figure.