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. 2020 Aug 10;21(10):e50219. doi: 10.15252/embr.202050219

Figure 5. Retinal neurodegeneration and gliosis in Tmem106b −/− Grn −/− mice.

Figure 5

  • A, B
    Retinal sections from 5‐month‐old mice were stained with indicated antibodies. Thickness of each layer, number of Brn3a‐positive cells (retinal ganglion cells) per section and length of outer segment as indicated by opsin staining are quantified.
  • C, D
    Increase in apoptosis in Grn −/− and Tmem106b −/− Grn −/− retina as indicated by TUNEL assay. TUNEL‐positive signals per section were quantified.
  • E, F
    Increase in Müller glial activation in Tmem106b −/− Grn −/− retina as indicated by GFAP staining. GFAP intensity per section was quantified and normalized to WT control.
  • G, H
    Increase in microglia activation in Grn −/− and Tmem106b −/− Grn −/− retina as indicated by IBA1 staining. The number of IBA1‐positive cells per section was quantified.
Data information: GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer. n = 3–6. Data presented as mean ± SEM. One‐way ANOVA tests with Bonferroni's multiple comparisons: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Scale bar = 50 μm.