Figure EV3. (related to Figs 1 and 3). Conversion of pRH6 into the naïve‐like state by 2a2iL under feeder‐free conditions.

• A
  The same protocol as Fig 1B was used for conversion of primed hESCs into naïve‐like cells without a feeder layer. Phase contrast morphology of 2a2iL‐RH6 and immunostaining for OCT4 and NANOG in nRH6 (left panel) and TFE3 and STAT3 indicated nuclear localization (right panel) scale bar: 50 μM.
• B
  Reversion of 2a2iL‐RH6 into the primed state (re‐primed RH6) with cytoplasmic localization of the TFE3 and STAT3 transcription factors.
• C
  Fluorescence images of tissue sections of non‐injected mouse in E10.5 (negative control group) showed no green or red signals representing GFP and human nuclear antigen (HNA) in different parts of the embryo.