Table 1.

Primer sequences and reaction conditions of qRT–PCR or PCR

Gene name	Primer (5′–3′)	Accession no.	Annealing temperature (°C)
qRT–PCR
GAPDH	F: ctc att tcc tgg tat gac aac ga	NM_001289746.1	60
	R: ctt cct ctt gtg ctc ttg ct
OCT4	F: ctg ggt tga tcc tcg gac ct	NM_002701.4	60
	R: cac aga act cat acg gcg gg
NANOG	F: aaa gaa tct tca cct atg cc	NM_024865.2	60
	R: gaa gga aga gga gag aca gt
SOX2	F: ggg aaa tgg aag ggg tgc aaa aga gg	NM_003106.3	60
	R: ttg cgt gag tgt gga tgg gat tgg tg
KLF4	F: att acc aag agc tca tgc ca	NM_004235.4	60
	R:cct tga gat ggg aac tct ttg
REX1	F: ttt acg ttt ggg agg agg	NM_174900.3	60
	R: gtg gtc agc tat tca gga g
DPPA3	F: aaa gct tcc gat aga ggg ga	NM_199286.3	60
	R: tgc tca ccg aag aaa att cc
TBX3	F: ttt gaa gac cat gga gcc cg	NM_005996.3	60
	R: aca ttc gcc ttc ccg act tg
KLF2	F: ggc aag acc tac acc aag ag	NM_016270	60
	R: cac aga tgg cac tgg aat gg
KLF5	F: cct ggt cca gac aag atg tga	NM_001730	60
	R: gaa ctg gtc tac gac tga ggc
DPPA5	F: gtc gtg gtt tac ggc tcc tat	NM_001025290	60
	R: ggc aag ttt gag cat ccc tc
MAEL	F: ttg ctg atg cca tcc ctt act	NM_032858	60
	R: agctgacatatctggaggtgaa
HORMAD1	F: gcc cag ttg cag agg act c	NM_001199829	60
	R: tct tgt tcc ata agc gca ttc t
TFCP2L1	F: cag ccc gag cac tac aac c	NM_014553	60
	R: ctc cca gct tcc gat tct cc
SOX1	F: cac aac tcg gag atc agc aa	NM_005986.2	60
	R: ggt act tgt aat ccg ggt gc
PAX6	F: gtc cat ctt tgc ttg gga aa	NM_001127612	60
	R: tag cca ggt tgc gaa gaa ct
TUBB3	F: gta tcc cga ccg cat cat	NM_006086	60
	R: tct cat ccg tgt tct cca
NESTIN	F: tcc agg aac gga aaa tca ag	NM_006617.1	60
	R: gcc tcc tca tcc cct act tc
GATA4	F: cct gtc atc tca cta cgg	NM_002052.3	60
	R: gct gtt cca aga gtc ctg
BRA	F: aat tgg tcc agc ctt gga at	NM_003181.2	60
	R: cgt tgc tca cag acc aca
CTNT	F: atg atg cat ttt ggg ggt ta	NM_000364.2	60
	R: cag cac ctt cct cct ctc ag
AFP	F: aaa tgc gtt tct cgt tgc tt	NM_001134.1	60
	R: gcc aca ggc caa tag ttt gt
FOXA2	F: gga gcg gtg aag atg gaa	NM_021784.4	60
	R: tac gtg ttc atg ccg ttc at
SOX17	F: ctc tgc ctc ctc cac gaa	NM_022454.3	60
	R: cag aat cca gac ctg cac aa
DNMT3β	F: tcc cag ctc tta cct tac ca	NM_001207056.1	60
	F: aaa ctc ctt ccc atc ctg ata ctc
TET1	F: cca acc tta ggg agt aac act g	NM_030625.2	60
	R: ggg agt gct gct tct ttc tg
DNMT3L	F: atg aag tca agg cta acc agc	NM_175867	60
	R: cgt cat cgt cgt aca gga aga g
KLF17	F: gtctttcttccgttctgatgag	NM_173484.3	60
	R: ttcttcattcacctaaggacca
SMAD2	F: aactatctcctactactctttccc	NM_005901.6	60
	R: cactcctcttcctatatgcct
SMAD3	F: agacaccagttctacctcct	NM_005902.4	60
	R: ggtctctggaatattgctctg
NODAL	F: gcg tac atg ctg agc ctc ta	NM_018055	60
	R: ggt gac ctg gga caa agt g
NR5A2	F: ctttgtcccgtgtgtggagat	NM_205860.3	60
	R: gtcggcccttacagcttcta
ARGFX	F: gaacgtacttctttcacccac	NM_001012659	60
	R: ttcctgaaccaaacctttactg
KHDC1L	F: gacttgatgacacgtaccttcg	NM_001126063.3	60
	R: agcgtgacacttggagtcct
LEFTY‐1	F: tgctacaggtgtcggtgcagagg	NM_020997.4	60
	R: agaaacggacacttgaaggccagg
LEFTY‐2	F: gggaattgggatacctggat	NM_003240.5	60
	R: ctaaatatgcacgggcaagg
PCR
Mitochondrial cytochrome C oxidase subunit I (COI)_Homo Sapiens	F: tagacatcgtactacacgacacg	‐	58
	R: tccaggtttatggagggttc

BRA, brachyury.