Table 2.
Primary and secondary antibodies used for immunofluorescence staining
| Antigen | Company | Cat no. | Dilution/concentration |
|---|---|---|---|
| OCT4 | Santa Cruz | Sc5279 | 1:200 |
| NANOG | Sigma‐Aldrich | N3038 | 1:500 |
| SOX2 | Santa Cruz | Sc20088 | 1:200 |
| TRA1‐81 | Invitrogen | 41‐1100 | 1:100 |
| SSEA1 | R&D System | MAB2155 | 1:250 |
| KLF4 | R&D System | Af3158 | 1:200 |
| TFE3 | Sigma‐Aldrich | HPA023881 | 1:200 |
| STAT3 | Cell Signaling | 4904S | 1:200 |
| TFCP2L1 | Sigma‐Aldrich | HPA029708 | 1:100 |
| KLF17 | Sigma‐Aldrich | HPA024629 | 1:100 |
| REX1 | Santa Cruz | Sc50671 | 1:100 |
| H3K27me3 | Abcam | Ab6002 | 1:500 |
| TUJ‐1 | Sigma‐Aldrich | T8660 | 1:200 |
| CTNT | Abcam | Ab64623 | 1:200 |
| SOX17 | R&D System | AF1924 | 1:100 |
| TBRA | R&D System | AF2085 | 1:100 |
| HNA | AbD Serotec | ab6970 | 1:200 |
| GAPDH | Proteintech | 60004‐1‐Ig | 1:30,000 |
| Anti‐mouse IgG‐Atto488 | Sigma‐Aldrich | 62197 | 1:500 |
| Anti‐mouse IgM‐Texas Red | Invitrogen | Sc2983 | 1:200 |
| Anti‐rabbit IgG‐Alexa Fluor 488 | Santa Cruz | A11008 | 1:500 |
| Anti‐goat IgG‐Alexa Fluor 488 | Invitrogen | A11055 | 1:500 |
| Anti‐rabbit IgG‐Alexa Fluor 546 | Invitrogen | A10040 | 1:500 |
| Anti‐mouse IgG‐Alexa 546 | Invitrogen | A10036 | 1:500 |
| Anti‐rabbit IgG‐peroxidase | Sigma‐Aldrich | A0545 | 1:1,000 |
| Anti‐goat TgG‐peroxidase | Sigma‐Aldrich | A5420 | 1:20,000 |
| Anti‐mouse IgG‐peroxidase | Sigma‐Aldrich | A2554 | 1:50,000 |
HNA, human nuclear antigen.