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. Author manuscript; available in PMC: 2020 Oct 5.
Published in final edited form as: Cell. 2019 Oct 17;179(3):713–728.e17. doi: 10.1016/j.cell.2019.09.020

Figure 2. Anatomic Distribution of VMH scRNA-Seq Clusters by seqFISH.

Figure 2.

(A) Schematic of seqFISH procedure in VMH. Light blue solid lines (1) outline ROIs of sequential hybridizations; yellow dashed lines (2) (maximum intensity Z projections) outline VMH and VMHvl. Three major anatomic regions (VMHvl, VMHc, and VMH-out) are color coded (3). Scale bars, 100 μm (inset).

(B) Heatmap showing expression level of marker genes (rows) in 27 seqFISH clusters (columns; n = 4,497; VMHvl only).

(C) Heatmaps showing correlation between seqFISH and SMART-seq clusters (n = 3,824; VMH only) and their p values (white, p > 0.05 or r < 0.35; see STAR Methods). seqFISH clusters that are the most strongly correlated with each scRNA-seq cluster are marked by red squares.

(D) Spatial distribution along A-P axis of seqFISH clusters (color-filled) showing anterior (#3, #5, #10) or posterior (#11, #26) biases, projected onto all segmented VMHvl cells (a–f). Line plot shows quantification for indicated clusters; black dotted line shows chance distribution. Scale bars, 50 μm (inset).

See also Figure S3.