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. 2020 Oct 1;218(1):e20200218. doi: 10.1084/jem.20200218

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© 2020 Paik and Farber

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Figure S1. — Primary infection generates influenza-specific lung T_RMs. (A) Lung cells isolated from mice infected 3–5 wk after primary infection. Left: Flow cytometry plots gated on lung CD44⁺CD8⁺ showing NP tetramer expression by lung niche (blue) and labeled (red) cells and graph showing number of lung CD44⁺CD8⁺NP-Tet⁺ cells. Right: Flow cytometry plots gated on medLN CD44⁺CD8⁺ showing CD103 and NP tetramer expression and graph depicting absolute number of medLN CD44⁺CD8⁺NP-Tet⁺ T cells. Data are representative of two experiments; n = 4 mice/group. (B) Lung niche T cells from memory mice express surface T_RM markers. Surface expression of CD69, CD103, and CXCR6 by lung niche (blue) versus circulating (labeled; red) T cells shown as representative histograms (left) and graphs showing individual mice (right) treated with FTY720 for 2 d. Data were compiled from two independent experiments; n = 7 mice/group. Significance was determined by Student’s unpaired t test; ****, P ≤ 0.0001; ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05. Error bars are mean ± SEM.