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. 2020 Sep 16;40(38):7203–7215. doi: 10.1523/JNEUROSCI.0443-20.2020

Figure 4.

Figure 4.

Progressive NMJ degeneration in HSA-Lmna−/− mice. A, B, NMJ morphology deficits in HSA-Lmna−/− mice. TA muscles were stained whole-mount with CF568 α-BTX (red) and anti-NF/Syn antibodies (visualized by Alexa Fluor 488, green). A, Representative images of TA muscles of control and mutant mice at different ages. Blue arrowheads indicated denervated or partially innervated endplates. Scale bar, 50 µm. B, Enlarged images of the individual NMJs. Scale bar, 20 µm. C, Progressive decrease in NMJ innervation in mutant mice. Data were shown as mean ± SEM, n = 5 mice per group. Control versus mutant: unpaired t test, t(8) = 3.515, **p = 0.0079 for 1 M; t(8) = 7.71, ***p < 0.0001 for 2 M; t(8) = 6.737, ***p = 0.0001 for 3 M; t(8) = 15.39, ***p < 0.0001 for 6 M. Control: one-way ANOVA, F(3,16) = 1.282, p = 0.3143. Mutant: one-way ANOVA, F(3,16) = 41.19; 2 versus 1 M, **p = 0.0078; 3 versus 2 M, *p = 0.0289; 6 versus 3 M, **p = 0.0033. D, Progressive NMJ fragmentation in mutant mice. Data were shown as mean ± SEM, n = 5 mice per group. Control versus mutant: unpaired t test, t(8) = 0.1127, p = 0.9131 for 1 M; t(8) = 4.725, **p = 0.0015 for 2 M; t(8) = 8.165, ***p < 0.0001 for 3 M; t(8) = 10.08, ***p < 0.0001 for 6 M. Control: one-way ANOVA, F(3,16) = 0.4222, p = 0.7397. Mutant: one-way ANOVA, F(3,16) = 47.74; 2 versus 1 M, **p = 0.0012; 3 versus 2 M, ***p = 0.0004; 6 versus 3 M, *p = 0.0159. E, Progressive reduction in AChR cluster area in mutant mice. Data were shown as mean ± SEM, n = 5 mice per group. Control versus mutant: unpaired t test, t(8) = 1.332, p = 0.2196 for 1 M; t(8) = 2.986, *p = 0.0175 for 2 M; t(8) = 4.883, **p = 0.0012 for 3 M; t(8) = 6.615, ***p = 0.0002 for 6 M. Control: one-way ANOVA, F(3,16) = 0.0835, p = 0.9680. Mutant: one-way ANOVA, F(3,16) = 36.62; 2 versus 1 M, *p = 0.04; 3 versus 2 M, *p = 0.0286; 6 versus 3 M, ***p = 0.0004. ns, no significant difference.