Figure 8.

Silencing of H2AX in human keratinocytes inhibits 53BP1 localization and causes DNA breaks. (a) Expression of γH2AX, 53BP1 or p-Res in CT or shH2AX expressing cells, (b) Quantitation of DNA comet tail lengths, in pixels, of CT or shH2AX expressing cells, n = 275 cells of 10 fields per slide. Treatment with DOXO was used as a severe DNA fragmentation control. Black small bars indicate the tail length average in each case. Photographs show representative comet average length for each condition. Data are mean ± SEM of duplicate (b) samples. Datasets were compared by an unpaired t test (two-sided). **, P < 0.01. Scale bar, 50 µm. Nuclear DNA by DAPI.