Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 1;219(11):e202001063. doi: 10.1083/jcb.202001063

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Molinuevo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S4. — Gain or loss of ATR activity affects terminal keratinocyte differentiation. (a and b) Expression of estrogen receptor (ER, red, a) or p-Res (red, b) in keratinocytes infected with control vector pMXPIE (CT) or with the TopB1P^ER construct, 48 h in the absence or presence of OHT. (c) γH2AX expression in keratinocytes as in a, by FC (+, positive keratinocytes according to negative isotype antibody control). (d) Phase contrast images of cells in a after 4 d in the presence of OHT, as indicated. (e) Phase contrast images of keratinocytes 4 d after infection with specific shATR or with the corresponding control vector plKO.1 (CT). (f) Light scatter parameters of keratinocytes 4 d after infection with shATR or with CT. Red box gates cells with HS typical of terminal differentiation. Scale bar, 50 µm. This figure complements Fig. 3 and Fig. 4. FSC, forward scatter; SSC, side scatter.