FIGURE 5.

CEACAM1 on NETs is important in adhesion of murine colon carcinoma cells to liver sinusoids in vivo. (A) Schematic depiction of the depletion reinfusion experiment. IVM, intravital microscopy; PMN, polymorphonuclear neutrophils. (B) Hepatic intravital video microscopy of CFSE-labeled MC38-CC1L in the liver sinusoids of neutrophil-depleted C57BL/6 mice (WT) reinfused with neutrophils from C57BL/6 mouse neutrophils either nonstimulated (first bar) or stimulated with 500 nM PMA (second bar) or with PMA and DNase 1 (1000 U) (third bar) or from CC1 KO mouse neutrophils either nonstimulated (fourth bar) or stimulated with PMA (fifth bar) or with PMA and DNase 1 (sixth bar). Shown are the mean and mean SE of five to seven experiments. *p < 0.05. (C) Representative images of the six conditions presented above with CFSE-labeled MC38-CC1L cells shown in green. Scale bar, 10 μm.