Table 1. PCR and nested PCR parameters.

Gene	Primers sequences	A	AT
Beta-actin*	F: 5’GGCATCCTGACCCTGAAGTA3’	98 bp	60º C
	R: 5’CGCAGCTCGTTGTAGAAGGT3’
BLV tax (extern)**	F: 5’GGCCCCACTCTCTACATGC3’	206 bp	57º C
	R: 5’AGACATGCAGTCGAGGGAAC3’
BLV tax (intern, nested)**	F: 5’ATGTCACCATCGATGCCTGG3’	113 bp	57º C
	R: 5’CATCGGCGGTCCAGTTGATA3’
BLV env (extern)**	F: 5’ TGATTGCGAGCCCCGATG 3’	230 bp	60º C
	R: 5’ GGAAAGTCGGGTTGAGGG 3’
BLV env (intern, semi-nested)	F: 5’ CCTCCCAGGCCGATCAAG 3’	165 bp	58º C

Genes and respective primer sequences, amplicon size (A) and annealing temperature (AT).

*Described by De Souza et al., 2018 [17]

** described by Buehring et al., 2014 [8].