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. 2020 Oct 5;14(10):e0008586. doi: 10.1371/journal.pntd.0008586

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© 2020 Fratini et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Biochemical characterization of EVs isolated from plasma pools from healthy subjects. (A) About 20% of sample volume from gradient fractions EXO/MV 5 was fixed for TEM analyses. (B) The diameter size distribution of particles is shown in percentage of total particles number and (C) in box-plot diagram. The median value of exosome diameter was 102 nm, while that of microvesicles was 158nm. (D) WB analysis of exosome markers CD81 (1:400), Synthenin (1:400), TFR 1 (1:1000), exposition time 1 min.