Table 3. Median quantification cycle values and estimates of yellow fever virus genomic load in liver samples of non-human primate carcasses.
| Variable | Median Cq (range) |
Median YFV genomic copies (range)/ gram of liver* | p-value** |
|---|---|---|---|
| Environment | p<0.0001 | ||
| Urban (n = 130) |
32.6 (7.6–37.0) |
5.96E+03 (1.74E+11–2.89E+02) |
|
| Urban-rural interface (n = 20) |
31.5 (9.2–36.2) |
1.27E+04 (5.78E+10–5.01E+02) |
|
| Rural (n = 148) |
13.0 (7.7–37) |
4.24E+09 (1.62E+11–2.89E+02) |
|
| Genera | p<0.0001 | ||
|
Alouatta (n = 41) |
13.0 (8.2–36.5) |
4.24E+09 (1.15E+11–4.08E+02) |
|
|
Callicebus (n = 22) |
8.5 (7.8–36.6) |
9.36E+10 (1.51E+11–3.81E+02) |
|
|
Callithrix (n = 210) |
32.0 (8.4–37.0) |
9.00E+03 (1.00E+11–2.89E+02) |
|
|
Environment/ genera |
p<0.0001 | ||
| Urban Callithrix (n = 120) |
33.0 (8.9–37) |
4.52E+03 (7.11E+10–2.89E+02) |
|
| Urban-rural Callithrix (n = 16) |
32.8 (9.2–36.2) |
5.19E+03 (5.78E+10–5.01E+02) |
|
| Rural Callithrix (n = 74) |
15.0 (8.4–37) |
1.07E+0. (1.00E+11–2.89E+02) |
|
| Rural Callicebus (n = 18) |
13.9 (7.8–37) |
2.28E+09 (1.51E+11–2.89E+02) |
|
| Rural Alouatta (n = 33) |
14.1 (8.3–36.5) |
1.99E+09 (1.07E+11–4.08E+02) |
n: number of carcasses. Cq: quantification cycle value. YFV: yellow fever virus. Liver samples of non-human primate carcasses collected in Minas Gerais state, Brazil (Jan 2017-Dec 2018), were tested for YFV RNA, using the RT-qPCR [24].
*Median Cqs were used to estimate YFV genomic copies per gram of liver using the equation: [10E (-0.2986 x Cq + 13.5094)].
**Median Cqs were compared using the Kruskal-Wallis test with Bonferroni correction for multiple comparisons with differences considered statistically significant at p< 0.0125. Analyses were run in R software v.3.6.0.