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Induction of reactive oxygen species (ROS) generation by Escherichia coli BAA 2471 (A), Enterobacter cloacae BAA 2468 (B), Klebsiella pneumoniae subsp. pneumoniae BAA 2472 (C), and Klebsiella pneumoniae BAA 2473 (D) was evaluated by DFCH-DA fluorimetric assay. Formation of ROS upon treatment with CSA-13, CSA-44, and CSA-131 at a concentration of 1–10 μg/mL was presented. Results show: mean±SD, n=3; * indicates statistical significance ≤0.05, ** ≤0.01, and *** ≤0.001.
