Figure 1.

Generation and validation of DPP3 knockout mice. A, strategy for the generation of DPP3-knockout mice. B and C, demonstration of the lack of DPP3 in male DPP3^−/− mice by Western blotting (B) and activity assays using Arg-Arg-2-naphthylamide as an artificial substrate (C). BAT, brown adipose tissue; SI, small intestine; SM, skeletal muscle; WAT, white adipose tissue (12–16 weeks of age; n = 3/group); n.d., non detectable. **, p < 0.01; ***, p < 0.001 versus WT mice based on unpaired two-sided Student's t test. The data are representative of three technical replicates from three biological replicates and presented as means ± S.D.