Table 1.
Kinetic parameters obtained from single-injection ITC experiments with hDPP3 and angiotensin peptides
The Michaelis–Menten constant (Km) and maximum turnover number (kcat) are obtained from the fit to a Michaelis–Menten curve for the reaction rate as a function of substrate concentration. The enthalpy of interaction (ΔH, also called heat of reaction) is a direct measurement of the rate at which heat is exchanged with the surroundings. The data represent the means ± S.D. All data points are three or more technical replicates from two biological replicates.
| Kinetic, calorimetric parameters of hDPP3 and angiotensin interaction |
|||
|---|---|---|---|
| ΔH | kcat | Km | |
| kcal/mol | s−1 | μm | |
| Ang II | −1.97 ± −0.06 | 0.25 ± 0.007 | 8.40 ± 0.40 |
| Ang(1–7) | −1.52 ± −0.10 | 0.14 ± 0.003 | 1.95 ± 0.20 |
| Ang(1–5) | −1.84 ± −0.04 | 0.35 ± 0.002 | 12.50 ± 2.20 |