Table 3.
Gi/o-mediated cAMP inhibition and β-arrestin-2 recruitment
Best fits were calculated with a variable slope from data performed in triplicate or quadruplicate from at least three independent experiments.
| Functional activity |
||||||
|---|---|---|---|---|---|---|
| CXCL12-WT |
CXCL12-P10Q |
CXCL12-P10del |
||||
| EC50 (pEC50 ± S.E.) | Emax | EC50 (pEC50 ± S.E.) | Emax | EC50 (pEC50 ± S.E.) | Emax | |
| nm | % CXCL12 | nm | % CXCL12 | nm | % CXCL12 | |
| CXCR4 | 0.03 (9.59 ± 0.20) | 100 | 4.90 (8.31 ± 0.18) | 90 ± 9 | 25.1 (7.60 ± 0.26) | 103 ± 16 |
| ACKR3 | 3.00 (8.52 ± 0.07) | 100 | 5.40 (8.27 ± 0.04) | 89 ± 2 | 38.1 (7.42 ± 0.09) | 80 ± 5 |