Figure 1.

A and B, liver Enho expression in B6 mice allowed unrestricted access to ND (A) (n = 56) or subject to CR (B) for 25 weeks (n = 47). C, heatmap of genes showing positive or negative correlations with Enho expression in the liver. The data shown in A–C are from young (Y, 19–29 weeks of age) or older (O, 55–69 weeks of age) mice subjected to ND or CR conditions. Liver samples were collected at the ZT indicated on the x axis (n = 3–6/group). Enho expression correlates positively with phosphoenolpyruvate carboxykinase (Pck1), which is rate-limiting for gluconeogenesis (D) but is incompatible with expression of genes involved de novo lipogenesis, such as fatty acid synthase (Fasn) (E). Note that the units of expression in D and E are z scores used for the heatmaps shown in C, whereas in A and B, Enho expression is shown as probe intensity. F, Enho expression relative to vehicle control in B6 mice 24 h after treatment with low, medium, or high doses of acetaminophen (APAP; at 169, 225, or 300 mg/kg), isoniazid (INZ; at 22, 44, or 88 g/kg), or paraquat (PQ; at 12.5, 25, or 50 mg/kg) (n = 5/group). Data in A–F were drawn from GSE93903, and data in G are from GSE51969. Correlation coefficients are shown for all data and for ND or CR separately. a, p < 0.05 versus ZT12, ZT16; b, p ≤ 0.05 versus ZT12, ZT16. In C, the heatmap was generated using z scores. In D and E, the values shown are z scores. *, p < 0.05 versus vehicle; **, p < 0.01 versus vehicle.