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. 2020 Jul 31;295(40):13798–13811. doi: 10.1074/jbc.RA120.014976

Figure 6.

Figure 6.

PDLIM5 interacts with SMAD3. A, co-immunoprecipitation analysis of the interaction between PDLIM5 and SMAD3 or SMAD2 in HEK293T cells. B, co-immunoprecipitation analysis of the endogenous interaction between PDLIM5 and SMAD3 in A549 cells. C, mapping PDLIM5 fragment that interacted with SMAD3. HEK293T cells were co-transfected with FLAG–SMAD3 and PDLIM5 truncated fragment (HA-PDLIM5, 1–596 amino acids; LIMs, 418–596 amino acids; △LIM, 1–417 amino acids; PDZ, 1–85 amino acids) for immunoprecipitation assays. D, mapping SMAD3 fragment that interacted with PDLIM5. HEK293T cells were co-transfected with HA-PDLIM5 and SMAD3 truncated fragment (FLAG–SMAD3, 1–425 amino acids; MH1, 1–137 amino acids; MH2, 231–425 amino acids) for immunoprecipitation assays. E, immunofluorescence analysis to show co-localization of SMAD3 (green) and PDLIM5 (red) in A549 cells. The cell nucleus is stained blue. Scale bar, 50 μm. IB, immunoblotting.