Figure 3.

Lysine 152 is a major site for FAAP20 acetylation. A, schematic representing FAAP20 and the two potential lysine residues for acetylation. The position of lysines 83 and 152, and conservation of their flanking regions are shown. B, acetylation of the FAAP20 lysine mutants. 293T cells expressing Flag–AAP20 WT or various lysine mutants were transfected with HA–CBP, and cell lysates were subjected to anti-Flag IP and Western blot using an acetyl-Lys specific antibody. C, quantification of FAAP20 acetylation levels in B. Error bar indicates mean ± S.D., n = 3 from three independent experiments, ****, p < 0.0001; ns, not significant, Student's t test.