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. 2020 Oct 5;9:e56749. doi: 10.7554/eLife.56749

Figure 6. Combined treatment inhibits xenograft growth and induces apoptosis in vivo.

SNU398 and MHCC97H cells were grown as tumor xenografts in BALB/c nude mice. Longitudinal tumor volume progression in SNU398 and MHCC97H tumor-bearing mice treated with vehicle (n = 6), CB-839 (150 mg/kg, oral gavage, twice per day; n = 6), V-9302 (30 mg/kg, intraperitoneal injection; n = 6), or combined therapies (n = 6) for 20 or 15 d, respectively. Growth curve and endpoint tumor volume of SNU398 (a, b) and MHCC97H (c, d) xenografts. (e, f) Representative images of HE, Ki67, cleaved caspase-3, and γH2AX in SNU398 (e) and MHCC97H (f) xenograft models. Scale bars = 50 μm. (g–i) Quantification of Ki67 positive cells (g), cleaved caspase-3 positive cells (h), and γH2AX positive cells (i) in SNU398 xenografts. (j–l) Quantification of Ki67 positive cells (j), cleaved caspase-3 positive cells (k), and γH2AX positive cells (l) in SNU398 xenografts. Data are represented as mean ± SEM. Statistical significance was assessed using a Student’s t test. *p<0.05, **p<0.01, ***p<0.001.

Figure 6—source data 1. Combined treatment inhibits xenograft growth and induces apoptosis in vivo.

Figure 6.

Figure 6—figure supplement 1. Combination of CB-839 and V-9302 showed no reduction of mice weight in vivo.

Figure 6—figure supplement 1.

SNU398 and MHCC97H tumor-bearing mice treated with vehicle (n = 6), CB-839 (150 mg/kg, oral gavage, twice per day; n = 6), V-9302 (30 mg/kg, intraperitoneal injection; n = 6), or combined therapies (n = 6) for 20 or 15 d, respectively. Graph shows mean ± SEM.