. 2020 Sep 21;9:e56359. doi: 10.7554/eLife.56359

Table 3. Effect of HD-tDCS on protein expression of dopaminergic neurotransmission factors in the prefrontal cortex, striatum, hippocampus, and SN/VTA (n = 5).

% of WKY	SHR	Sham	tDCS-PFC	tDCS-M1	MPH	Sham vs. tDCS-PFC	Sham vs. tDCS-M1	Sham vs. MPH	PFC vs. M1
Prefrontal cortex
TH	53.52 ± 31.75	82.28 ± 43.03	100.94 ± 26.54	98.30 ± 43.88	65.79 ± 18.48	p=0.43	p=0.58	p=0.45	p=0.91
TH	53.52 ± 31.75	82.28 ± 43.03	100.94 ± 26.54	98.30 ± 43.88	65.79 ± 18.48	F(1,8)=0.68	F(1,8)=0.34	F(1,8)=0.62	F(1,8)=0.01
DAT	89.41 ± 52.58	108.69 ± 31.93	89.63 ± 39.41	83.32 ± 32.20	86.40 ± 27.72	p=0.43	p=0.25	p=0.27	p=0.79
DAT	89.41 ± 52.58	108.69 ± 31.93	89.63 ± 39.41	83.32 ± 32.20	86.40 ± 27.72	F(1,8)=0.70	F(1,8)=1.56	F(1,8)=1.39	F(1,8)=0.08
VMAT2	71.61 ± 48.26	57.09 ± 24.08	76.33 ± 20.62	71.56 ± 40.23	70.45 ± 29.33	p=0.21	p=0.51	p=0.45	p=0.82
VMAT2	71.61 ± 48.26	57.09 ± 24.08	76.33 ± 20.62	71.56 ± 40.23	70.45 ± 29.33	F(1,8)=1.84	F(1,8)=0.48	F(1,8)=0.62	F(1,8)=0.06
Striatum
TH	103.70 ± 15.14	95.96 ± 11.46	101.56 ± 22.61	91.96 ± 11.52	103.76 ± 21.11	p=0.63	p=0.55	p=0.49	p=0.42
TH	103.70 ± 15.14	95.96 ± 11.46	101.56 ± 22.61	91.96 ± 11.52	103.76 ± 21.11	F(1,8)=0.24		F(1,8)=0.53	F(1,8)=0.72
DAT	117.76 ± 37.47	101.44 ± 34.52	120.34 ± 71.05	101.35 ± 47.65	107.57 ± 34.41	p=0.61	p=1.0	p=0.79	p=0.63
DAT	117.76 ± 37.47	101.44 ± 34.52	120.34 ± 71.05	101.35 ± 47.65	107.57 ± 34.41	F(1,8)=0.29		F(1,8)=0.08	F(1,8)=0.25
VMAT2	90.46 ± 57.19	66.67 ± 38.56	71.25 ± 35.22	85.87 ± 56.83	73.78 ± 25.00	p=0.85	p=0.55	p=0.74	p=0.64
VMAT2	90.46 ± 57.19	66.67 ± 38.56	71.25 ± 35.22	85.87 ± 56.83	73.78 ± 25.00	F(1,8)=0.04	F(1,8)=0.39	F(1,8)=0.12	F(1,8)=0.24
Hippocampus
TH	79.55 ± 40.22	111.39 ± 31.31	78.36 ± 45.70	96.29 ± 52.81	78.48 ± 46.69	p=0.22	p=0.60	p=0.23	p=0.58
TH	79.55 ± 40.22	111.39 ± 31.31	78.36 ± 45.70	96.29 ± 52.81	78.48 ± 46.69	F(1,8)=1.78	F(1,8)=0.30	F(1,8)=1.71	F(1,8)=0.33
DAT	64.33 ± 35.74	159.83 ± 87.35	87.51 ± 56.22	152.10 ± 68.80	77.47 ± 28.67	p=0.16	p=0.88	p=0.08	p=0.14
DAT	64.33 ± 35.74	159.83 ± 87.35	87.51 ± 56.22	152.10 ± 68.80	77.47 ± 28.67	F(1,8)=2.42	F(1,8)=0.024	F(1,8)=4.01	F(1,8)=2.64
VMAT2	81.40 ± 30.60	88.84 ± 26.80	113.49 ± 33.17	91.26 ± 17.67	117.83 ± 22.95	p=0.23	p=0.87	p=0.10	p=0.22
VMAT2	81.40 ± 30.60	88.84 ± 26.80	113.49 ± 33.17	91.26 ± 17.67	117.83 ± 22.95	F(1,8)=1.67	F(1,8)=0.028	F(1,8)=3.38	F(1,8)=1.75
SN/VTA
TH	84.35 ± 48.89	68.41 ± 30.38	94.69 ± 34.28	120.88 ± 31.89	142.75 ± 58.04	p=0.24	p=0.03	p=0.04	p=0.25
TH	84.35 ± 48.89	68.41 ± 30.38	94.69 ± 34.28	120.88 ± 31.89	142.75 ± 58.04	F(1,8)=1.65	F(1,8)=7.10	F(1,8)=6.44	F(1,8)=1.56
DAT	56.52 ± 27.67	96.48 ± 21.33	40.37 ± 29.72	56.96 ± 27.14	72.24 ± 47.04	p=0.01	p=0.03	p=0.33	p=0.38
DAT	56.52 ± 27.67	96.48 ± 21.33	40.37 ± 29.72	56.96 ± 27.14	72.24 ± 47.04	F(1,8)=11.76	F(1,8)=6.56	F(1,8)=1.10	F(1,8)=0.85
VMAT2	56.87 ± 33.03	74.15 ± 31.39	79.95 ± 25.70	53.48 ± 17.09	57.24 ± 28.47	p=0.76	p=0.23	p=0.40	p=0.09
VMAT2	56.87 ± 33.03	74.15 ± 31.39	79.95 ± 25.70	53.48 ± 17.09	57.24 ± 28.47	F(1,8)=0.10	F(1,8)=1.67	F(1,8)=0.80	F(1,8)=3.68

Expression of the TH, DAT, and VMAT2 proteins, expressed as percentages of WKY. Data are presented as the mean ± SEM. Data were analyzed using ANOVA for repeated measures, followed by Tukey's tests for multiple comparisons. p<0.05 was considered statistically significant; significant results are highlighted in bold.