Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | CAG:GFP-Centrin2 | PMID:21752934 | RRID:MGI:3793421 | Xiaowei Lu (University of Virginia) |
| Genetic reagent (M. musculus) | Atoh1-Cre | PMID:19609565 | RRID:MGI:3775845 | Bernd Fritzsch (University of Iowa) |
| Genetic reagent (M. musculus) | Gfi1-Cre | PMID:20533399 | RRID:MGI:4430258 | Lin Gan (Augusta University) |
| Genetic reagent (M. musculus) | Rosa26REmx2 | PMID:28266911 | ||
| Genetic reagent (AAV-Virus) | AAV2.7m8-CAG-Emx2-P2A-tdTomato | Vector Biolabs | 4.0 × 1010 GC in 100 µl culture medium | |
| Genetic reagent (AAV-Virus) | AAV2.7m8-CAG-tdTomato | Vector Biolabs | 4.0 × 1010 GC in 100 µl culture medium | |
| Chemical compound, drug | nocodazole | Sigma-Aldrich | M1404 | 5 μM |
| Chemical compound, drug | pertussis toxin | Millipore Sigma | 516560–50 UG | 8.4 nM |
| Chemical compound, drug | SiR-tubulin | Spirochrome | SC002 | 1 μM |
| Antibody | Mouse anti-βII spectrin | BD Biosciences | Cat# 612562, RRID:AB_399853 | IHC (1:500) |
| Antibody | Rabbit anti-Arl13b | Proteintech | Cat# 17711–1-AP, RRID:AB_2060867 | IHC (1:500) |
| Antibody | Mouse anti-acetylated tubulin | Sigma-Aldrich | Cat# T7451, RRID:AB_609894 | IHC (1:500) |
| Antibody | Rabbit anti-ninein | Abcam | Cat# Ab231181 | IHC (1:500) |
| Antibody | Rabbit anti-γ-tubulin | Sigma Aldrich | Cat# ab4447, RRID:AB_304460 | IHC (1:500) |
| Antibody | Rabbit anti-Emx2 | Trans Genic | Cat# KO609 | IHC (1:250) |
| Antibody | Rabbit anti-Gαi | Provided by B. Nurnberg | IHC (1:1000) | |
| Antibody | Alexa Fluor 488 donkey anti-mouse IgG | Thermo Fisher Scientific | Cat# A-21202 RRID:AB_2535788 | IHC (1:500) |
| Antibody | Alexa Fluor 647 donkey anti-mouse IgG | Thermo Fisher Scientific | Cat# A-31571 RRID:AB_162542 | IHC (1:500) |
| Antibody | Alexa Fluor 405 Donkey anti rabbit IgG | Abcam | ab175651 | IHC (1:500) |
| Antibody | Alexa Fluor donkey anti-rabbit 647 | Thermo Fisher Scientific | Cat# A-31573 RRID:AB_2536183 | IHC (1:500) |
| Software, algorithm | ImageJ | RRID:SCR_003070 | ||
| Software, algorithm | Imaris 9.5.0 | Bitplane | RRID:SCR_007370 |