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. 2020 Sep 7;9:e58149. doi: 10.7554/eLife.58149

Figure 5. Ppl and Evpl domain localization.

(A) Periderm cells expressing Evpl-tdTomato and Ppl-GFP variants at 48hpf. Schematics indicate the domains in each variant. (B–C) Optical sections of GFP-tagged Ppl truncated rod-tail fusions expressed in periderm cells at 48hpf. Sections highlight the microridge-like pattern at the apical surface of cells (left, red outlines) or the filamentous pattern deeper in cells (right, blue outlines). Top: Diagram of Ppl protein domains. Amino acid numbers are indicated. See Figure 5—video 1 for video showing apical-to-basal sections of these images. Images were inverted so that high-intensity fluorescence appears black and low-intensity fluorescence is white. Scale bars: 10 µm.

Figure 5.

Figure 5—figure supplement 1. Evpl depends on Ppl for apical localization, but not vice versa.

Figure 5—figure supplement 1.

(A) Ppl-GFP[BAC]- and Lifeact-mRuby-expressing WT and evpl−/− periderm cells. Ppl localized to microridges in WT and pegs/short microridges in evpl−/− periderm cells at 48hpf. (B) Evpl-GFP[BAC]- and Lifeact-mRuby-expressing WT and ppl−/− periderm cells at 48hpf. Evpl localized to microridges in WT and to the cytoplasm in ppl−/− cells. Black-and-white images were inverted so that high-intensity fluorescence appears black and low-intensity fluorescence is white. Scale bars: 10 µm.
Figure 5—video 1. Truncated periplakin rod-tail domains localize to microridges and the IF filamentous network.
Download video file (2.8MB, mp4)
Video showing apical-to-basal confocal optical sections of periderm cells expressing GFP tagged Ppl(sRT1) and Ppl(sRT2) in WT animals, as indicated by title cards. Scale bars: 5 μm.