Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (zebrafish; Danio rerio) |
Tg(krt5:Lifeact-GFP) | van Loon et al., 2020 | la226Tg | |
| Genetic reagent (zebrafish; Danio rerio) |
Tg(Krt5:Lifeact-Ruby) | van Loon et al., 2020 | la227Tg | |
| Genetic reagent (zebrafish; Danio rerio) |
Tg(UAS:eGFP-PH-PLC), a.k.a Tg(4xuAS:UAS:eGFP-Rno-Plcd1) |
Jiang et al., 2019 | la216Tg | |
| Genetic reagent (zebrafish; Danio rerio) |
Envoplakin mutant | See Figure 4, this paper | ||
| Genetic reagent (zebrafish; Danio rerio) |
Periplakin mutant | See Figure 4, this paper | ||
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-211 BAC library | Ch211-188J19 | BAC for creating Envoplakin reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-360K2 | BAC for creating Periplakin reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-107M18 | BAC for creating Krt17 reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-294H4 | BAC for creating Krt99 reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-107M18 | BAC for creating Krt92 reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-107M18 | BAC for creating krt1-19d reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-107M18 | BAC for creating cyt1 reporter |
| Recombinant DNA reagent | Bacterial artificial chromosome | CHORI-73 BAC library | Ch73-107M18 | BAC for creating cyt1l reporter |
| Sequence-based reagent | Morpholino antisense oligonucleotide targeting Ppl |
Gene Tools, LLC | 5’-TCTGAGGTGAAACAACAGCGAGTTT-3 | |
| Sequence-based reagent | Morpholino antisense oligonucleotide targeting Evpl |
Gene Tools, LLC | 5’-GTGTCTTTAGTGTCACTCACTCATT-3’ | |
| Sequence-based reagent | Genotyping Evpl wild-type and mutant allele; forward primer |
Fisher Scientific | 5’-AGAAGAGCTGCTGCTCCTTG-3’ | |
| Sequence-based reagent | Genotyping Evpl wild-type allele; reverse primer |
Fisher Scientific | 5’-CTCATGAAGGCGACTTACAC-3’ | |
| Sequence-based reagent | Genotyping Evpl mutant allele; reverse primer |
Fisher Scientific | 5’-TATTGTCTGCGAACGTCAGC-3’ | |
| Sequence-based reagent | Genotyping Ppl wild-type and mutant allele; forward primer |
Fisher Scientific | 5’-TTCCCTCAGCAGATGATGCG-3’ | |
| Sequence-based reagent | Genotyping Ppl wild-type allele; reverse primer |
Fisher Scientific | 5’-CTCAGCAAGAGAGCCGACAC-3’ | |
| Sequence-based reagent | Genotyping Ppl mutant allele; reverse primer |
Fisher Scientific | 5’-CCAGTAGTCTGTTGTACCTCGC-3’ | |
| Sequence-based reagent | Forward primer for evpl exon3 gRNA synthesis |
Fisher Scientific | 5’-GCGTAATACGACTCACTATAGG
CAGCAGATCTCCTTGGCCGTTTT AGAGCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Forward primer for evpl exon7 gRNA synthesis |
Fisher Scientific | 5’-GCGTAATACGACTCACTATAGGA
GGAGGTGGCATACATGAGTTTT AGAGCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Forward primer for ppl exon2 gRNA synthesis |
Fisher Scientific | 5’-GCGTAATACGACTCACTATAGGCG
CCAAATCAGTTTCGGGGTTTT AGAGCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Forward primer for ppl exon5 gRNA synthesis |
Fisher Scientific | 5’-GCGTAATACGACTCACTATAGGATA
GTGAAGTGGAGGCACGTTTT AGAGCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Forward primer for Ppl-GFP homologous recombination gRNA synthesis |
Fisher Scientific | 5’-CGCTAATACGACTCACTATAGGGGTCTATTATATGCGGTCTAGTTTTAGAGCTAGAAATAGC-3’ | |
| Sequence-based reagent | Forward primer for Krt17-GFP homologous recombination gRNA synthesis |
Fisher Scientific | 5’-CGCTAATACGACTCACTATAGGGTGG
CGATTCATTCCGTTGGTTTT AGAGCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Forward primer of donor plasmid gRNA synthesis for homologous recombination |
Fisher Scientific | 5’-CGCTAATACGACTCACTATAGGATAGTG
AAGTGGGTAGATGGACCGGGGGTTTTAGA GCTAGAAATAGC-3’ |
|
| Sequence-based reagent | Constant reverse primer for gRNA synthesis |
Fisher Scientific | 5’-AAAGCACCGACTCGGTGCCACTTTTT
CAAGTTGATAACGGACTAGCCTTATTTTAA CTTGCTATTTCTAGCTCTAAAAC-3’ |
|
| Sequence-based reagent | Forward primer for Ppl homology arm upstream of the stop codon |
Fisher Scientific | 5’-GGGGACAACTTTGTATAGAAAGTTGGCGCGGCTTAATGAACTTG-3’ | |
| Sequence-based reagent | Reverse primer for Ppl homology arm upstream of the stop codon |
Fisher Scientific | 5’-GGGGACTGCTTTTTTGTACAAA
CTTGCTTTGCCTCTGCCTGACA-3’ |
|
| Sequence-based reagent | Forward primer for Ppl homology arm downstream of the stop codon |
Fisher Scientific | 5’-GGGGACAGCTTTCTTGTACAAA
GTGGGATCACCACTTGACCTGTG-3’ |
|
| Sequence-based reagent | Reverse primer for Ppl homology arm downstream of the stop codon |
Fisher Scientific | 5’-GGGGACAACTTTGTATAATAAA
GTTGCTTAATTGTTTATGTGGTATCC-3’ |
|
| Sequence-based reagent | Forward primer for Krt17 homology arm upstream of the stop codon |
Fisher Scientific | 5’-GGGGACAACTTTGTATAGAAA
GTTGGCATTCATTAATAGTTCACATTC-3’ |
|
| Sequence-based reagent | Reverse primer for Krt17 homology arm upstream of the stop codon |
Fisher Scientific | 5’-GGGGACTGCTTTTTTGTA
CAAACTTGCAGTGGTTTTAGTCTGGC-3’ |
|
| Sequence-based reagent | Forward primer for Krt17 homology arm downstream of the stop codon |
Fisher Scientific | 5’-GGGGACAGCTTTCTTGTACAAA
GTGGGAACTATCTGTAGCATCTCG-3’ |
|
| Sequence-based reagent | Reverse primer for Krt17 homology arm downstream of the stop codon |
Fisher Scientific | 5’-GGGGACAACTTTGTATAATAAA
GTTGCCTTTGGACTGTAAGGGA-3’ |
|
| Sequence-based reagent | Forward primer of Evpl head domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAAGCA
GGCTTTATGTTCAAGAGGAATAAAGACA-3’ |
|
| Sequence-based reagent | Reverse primer of Evpl head domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAAGCT
GGGTAGAGAACTTCAGTGTCCTCAAAG-3’ |
|
| Sequence-based reagent | Forward primer of Evpl rod domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTTTATGCAACAGCAGCTGC-3’ |
|
| Sequence-based reagent | Reverse primer of Evpl rod domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTATGTGGAAACATT GACTTCTTTTG-3’ |
|
| Sequence-based reagent | Forward primer of Evpl tail domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTTTATGGACTCCTCTGAGATGCG-3’ |
|
| Sequence-based reagent | Reverse primer of Evpl tail domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTAGACATTTTTTGAGG AAGCATTAAACATCG-3’ |
|
| Sequence-based reagent | Forward primer of Ppl head domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTTTATGTTCAAGAAAA GGCAAACCA-3’ |
|
| Sequence-based reagent | Reverse primer of Ppl head domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTACTCATCCAGTT GAGGGTCCGGC-3’ |
|
| Sequence-based reagent | Forward primer of Ppl rod domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTTTATGAAGGTG CCGGACCCTCAAC-3’ |
|
| Sequence-based reagent | Reverse primer of Ppl rod domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTACACTCGCATTGC AGCCAGACTC-3’ |
|
| Sequence-based reagent | Forward primer of Ppl tail domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTTTATGAAGAGTCT GGCTGCAATGC-3’ |
|
| Sequence-based reagent | Reverse primer of Ppl tail domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTATTTGCCTCTGCC TGACAGCATG-3’ |
|
| Sequence-based reagent | Forward primer of Ppl short rod-tail1 domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTGGATGGATGCGGA GCTCGAAATTCAG-3’ |
|
| Sequence-based reagent | Forward primer of Ppl short rod-tail2 domain |
Fisher Scientific | 5’-GGGGACAAGTTTGTACAAAAAA
GCAGGCTGGATGATCACAACAAC AGAAACCAGACAC-3’ |
|
| Sequence-based reagent | Constant reverse primer of Ppl short rod-tail domain |
Fisher Scientific | 5’-GGGGACCACTTTGTACAAGAAA
GCTGGGTAGACATTTTTTGAGG AAGCATTAAACATCG-3’ |
|
| Sequence-based reagent | Forward primer of Ppl ∆DWEEI | Fisher Scientific | 5’-ACCGTCAAGGGTCCAAGTGGC-3’ | |
| Sequence-based reagent | Reverse primer of Ppl ∆DWEEI | Fisher Scientific | 5’-GCACTCTTGACTTTGAA
GGTTGACAAAC-3’ |
|
| Sequence-based reagent | Forward primer of Ppl RT-PCR | Fisher Scientific | 5’-AACCAAGAGTAGCGCGACCATC-3’ | |
| Sequence-based reagent | Reverse primer of Ppl RT-PCR | Fisher Scientific | 5’-AGTAGTCTGTTGTACCTCGCC
−3’ |
|
| Sequence-based reagent | Forward primer of Epl RT-PCR | Fisher Scientific | 5’-TGAGAAAGATGTACTGCGCG
−3’ |
|
| Sequence-based reagent | Forward primer of Epl RT-PCR | Fisher Scientific | 5’-CGTCAGCATGGTCGACCATC-3’ | |
| Sequence-based reagent | Forward primer of βactin RT-PCR | Fisher Scientific | 5’-CGTGACATCAAGGAGAAGCT-3’ | |
| Sequence-based reagent | Forward primer of βactin RT-PCR | Fisher Scientific | 5’-ATCCACATCTGCTGGAAGGT-3’ | |
| Peptide, recombinant protein | Alt-R S.p. Cas9 Nuclease 3NLS | IDT | 1074182 | |
| Peptide, recombinant protein | Platinum SuperFi DNA Polymerase |
Invitrogen | 12351–010 | |
| Chemical compound, drug | CK666, Tocris Bioscience | Fisher Scientific | 395050 | |
| Chemical compound, drug | Alexa Fluor 488 Phalloidin | Thermo Fisher Scientific | A12379 | |
| Commercial assay or kit | HiScribe T7 High Yield RNA Synthesis Kit |
New England Biolabs | E2040S | |
| Commercial assay or kit | RNeasy Mini Kit | QIAGEN | 74104 | |
| Software, algorithm | Zen2.1(Blue edition) | Carl Zeiss Microscopy | http://www.zeiss.com | |
| Software, algorithm | FIJI/ImageJ | Schindelin et al., 2012 | https://fiji.sc/ | |
| Software, algorithm | RStudio | R Foundation for Statistical Computing |
https://www.r-project.org/ |