Skip to main content
. Author manuscript; available in PMC: 2020 Oct 6.
Published in final edited form as: Biomaterials. 2020 Feb 14;240:119856. doi: 10.1016/j.biomaterials.2020.119856

Figure 1. Fabrication of anisotropic multilayered tissues using flexible thermoresponsive nanofabricated stubstrates (fTNFS) and nanopatterned cell sheet engineering.

Figure 1.

A. Fabrication of flexible nanopatterned substrates (fTNFS) using capillary force lithography and subsequent thermoresponsive functionalization with amine-terminated PNIPAM (a-PNIPAM).

B. Image of flexible-TNFS after curing and a-PNIPAM functionalization. Rainbow coloring is caused by the nanotopography deflecting light. (Inset) Scanning electron micrograph of fTNFS surface demonstrating high fidelity fabrication of the ridge-groove nanotopography.

C. Schematic of gel casting and stacking of organized cell monolayers from flexible TNFS.

D. Z-stack cross-sectional image of smooth muscle cell tri-layer tissue stack 24 hours after stacking. Top and bottom sheets were membrane-dyed red (Cell Tracker Red) and middle sheet was membrane-dyed green (Cell Tracker Green) before stacking.