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. 2020 Oct 5;11:4980. doi: 10.1038/s41467-020-18735-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Induced knockdown of MILIP prolonged the half-life time of p53 protein in cycloheximide (CHX)-chase assays. Data are representatives or mean ± s.d.; n = 3 independent experiments, two-tailed Student’s t test. CHX: 40 µg/mL, DOX: 200 ng/mL. b MILIP silencing reduced p53 polyubiquitination. Data shown represent three independent experiments. c Induced knockdown of MILIP did not reduce the binding between p53 and MDM2. Data shown represent three independent experiments. d MILIP was coprecipitated with p53 using RNA immunoprecipitation (RIP) assays. Data shown represent three independent experiments. e p53 was co-pulled down with MILIP by antisense probes against MILIP using RNA pulldown assays. Data shown represent three independent experiments. f In vitro transcribed MILIP was coprecipitated with purified Flag-tagged p53 in a cell-free system using RNA immunoprecipitation (RIP) assays. Data shown represent three independent experiments. g Overexpression of MILIP but not a MILIP mutant with the −966/−1199 segment deleted (MILIP Δ−966/−1199) downregulated both endogenous (left) and overexpressed exogenous (right) p53 protein levels. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA followed by Tukey’s multiple comparisons test. Source data of Fig. 3a–g are provided as a Source Data file.