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. 2020 Oct 5;11:4977. doi: 10.1038/s41467-020-18701-4

Fig. 4. IL6 trans-signaling regulates sphere-forming ability.

Fig. 4

a MCF 10A cells were cultured as spheres in the absence (n = 18) or presence of IL6 (n = 18), an IL6-blocking antibody (n = 6) or hyper-IL6 (n = 6). b hTERT-HME1 were cultured as spheres in the absence (n = 3) or presence (n = 3) of hyper-IL6. c HMECs were cultured as spheres without or with IL6, with an IL6 blocking antibody or hyper-IL6. n = 3 patients, each patient analyzed in triplicate. d hTERT-HME1-EGFR∆746-750 cells were cultured as spheres in the absence or presence of HIL6 (each n = 12). e MCF 10A cells were cultured as spheres without (n = 6) or with IL6 (n = 6) and IL6 plus sgp130-Fc at indicated concentrations (each n = 6). f Sphere formation of hTERT-HME1-EGFR∆746-750 in the absence (n = 10) or presence of an anti-IL6 antibody (n = 9) or with sgp130-Fc at indicated concentrations (each n = 12). Cumulative data of three experiments. P values in a, c, f: one-way ANOVA with Dunnett’s multiple comparisons test (post hoc); b, d two-sided Student’s t-test; e one-way ANOVA with Tukey’s multiple comparisons test (post hoc); asterisks indicate significance between groups (****p < 0.0001). All error bars correspond to standard deviation (Mean ± SD). See Supplementary Table 1 for patient/sample-ID allocation.